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KMID : 0903520090520010001
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2009 Volume.52 No. 1 p.1 ~ p.10
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Isolation of Sporidiobolus ruineniae CO-3 and Characterization of Its Extracellular Protease
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Kim Ji-Yeon
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Abstract
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An extracellular protease-producing yeast strain, designated as CO-3, was isolated from fermented tea. The cells were spherical- to ovoid-shaped and 6.8-7.4×7.4-9.1 μm in size. Optimal growth conditions were 25-30 ℃ and pH of 5.0-6.0. The isolate was able to grow in up to 4%(w/v) NaCl and 5%(v/v) ethanol. This strain was identified as Sporidiobolus ruineniae based on the internal transcribed spacer regions including 5.8S rDNA sequence and partial D1/D2 domain of 26S rDNA sequence analysis. Optimal activity conditions of the crude protease fraction of S. ruineniae CO-3 were pH of 7.0 at 50℃. Protease production reached maximum when 1.0%(w/v) xylose, 1.0%(w/v) yeast extract, and 0.3%(w/v) K2HPO4 were used as the sole sources of carbon, nitrogen, and mineral, respectively.
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KEYWORD
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extracellular protease, identification, molecular phylogeny, Sporidiobolus ruineniae, yeast
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